KRAS Real-time PCR Kit

Test Overview

The KRAS PCR Kit (K-RAS Kit) is an in vitro diagnostic test intended for the qualitative detection of KRAS somatic mutations in genomic DNA extracted from fresh, frozen or formalin-fixed paraffin-embedded (FFPE) tissue. The TRUPCR® KRAS PCR Kit is based on allele-specific amplification and is achieved by ARMS PCR. Taq DNA polymerase is extremely effective at distinguishing between a match and a mismatch at the 3’-end of a PCR primer.

The TRUPCR® KRAS Real-time PCR Kit is designed to selectively amplify mutant-specific sequences in samples that contain a mixture of wild-type and mutated DNA. The most common mutations are found in codons 12, 13 and 61. The detection is achieved using fluorescent probes labelled with FAM and HEX. The TRUPCR® KRAS PCR Kit is composed of 11 assays for the detection of the KRAS mutations and a reference control gene of KRAS region without any known polymorphism / mutation.

Key Features of the KRAS Real-time PCR Kit:

Thiopurine methyltransferase (TPMT) is an enzyme crucial for metabolizing thiopurine drugs such as azathioprine, mercaptopurine, and thioguanine. These drugs are widely used to treat leukaemia, inflammatory bowel disease (IBD), and autoimmune disorders. TPMT activity varies among
individuals due to genetic differences, which significantly affects how these drugs are processed in the body. People with normal TPMT activity can typically tolerate standard doses, while those with intermediate activity may need dose adjustments to avoid side effects. Individuals with low or
absent TPMT activity are at high risk for severe myelosuppression and require much lower doses or alternative treatments.

Testing for TPMT activity before starting thiopurine therapy is recommended to personalize treatment and minimize the risk of adverse effects, making TPMT testing a key component of personalized medicine