BCR ABL Qualitative qPCR Testing Kit

Test Overview

The BCR-ABL1 Qualitative Detection qPCR Testing Kit is intended for the qualitative detection of BCR-ABL1 fusion gene (Major-BCR, minor-BCR, and micro-BCR) in bone marrow or peripheral blood samples using a Real-time PCR system. Our BCR ABL Qualitative qPCR Testing Kit is based on reverse transcription of total RNA, followed by Real-time PCR amplification and detection of BCR-ABL1 (e13a2, e14a2 or e1a2 or e19a2) fusion transcripts and the ABL transcript.

Other Kits Available

We have a few other products in our BCR-ABL range that might interest you:

TRUPCR® BCR-ABL Quantitative Kit
TRUPCR® BCR-ABL1 b2a2/b3a2 Differentiation Kit
TRUPCR® BCR-ABL1 Transcript Screening Kit

Principle and Procedure

The TRUPCR® BCR-ABL1 Qualitative Detection qPCR Testing Kit is a real-time amplification test for the detection of BCR-ABL1 e13a2, e14a2, e1a2 and e19a2 fusion transcripts in bone marrow or peripheral blood samples. It has a two-step protocol in which total RNA is reverse-transcribed, and the generated cDNA is amplified by PCR. To control sample quality, the kit uses the RNA detection of ABL. Amplification of this control RNA is visualized in the FAM channel in a separate tube.

Key Features

CE-IVD
Detection and differentiation of all three Major, Minor and Micro transcripts.
All-inclusive kit: The Assay includes cDNA preparation components and all the PCR components including PCR master mix for optimized results.
No need to standardize your own cDNA prep which may lead to variability in the results.
Detection up to 1 copy of BCR ABL1 transcript.