BRAF Mutation Kit

The BRAF Mutation Kit is an in vitro diagnostic test intended for the qualitative detection of BRAF somatic mutations in genomic DNA extracted from fresh, frozen or formalin fixed paraffin-embedded (FFPE) tissue. The TRUPCR® BRAF mutation kit is based on allele specific amplification and is achieved by ARMS PCR. Taq DNA polymerase is extremely effective at distinguishing between a match and a mismatch at the 3’-end of a PCR primer. The kit is designed to selectively amplify mutant specific sequences in samples that contain a mixture of wild-type and mutated DNA. The most common mutations are found in codon 600. The detection is achieved using fluorescent probes labelled with FAM and HEX. The TRUPCR® BRAF Kit is composed of 7 assays for the detection of the BRAF mutations and a reference control gene of BRAF region without any known polymorphism/mutation.