Coincidence of references
By using National reference panel for the complete genome of Human Papillomavirus genotyping to evaluate this kit, the results were consistent with both positive references and negative references. For enterprise references, the coincidence rates of 11 negative references and 24 positive references are both 100%.
The limit of detection (LoD)
The limit of detection for all targeted HPV types are 500 copies/mL-1.
Precision
The coefficient of variation of Ct values of precision references is not higher than 5% within-batch and inter-batch.
Interfering substances
10% white blood cells, 5% lubricant, 2% antibacterial lotion, 2mg/mL-1 hemoglobin, 0.1mg/mL-1 of mucin,10mg/mL-1 benzene ether embolism or 4mg/mL-1 nitric acid contained in the sample had no significant influence on the performance of the kit. However, still try to avoid any interference substances in the process of sampling.